dooa عضو مميز
عدد المساهمات : 270 العمر : 37 تاريخ التسجيل : 05/08/2008
| موضوع: كيفية اجراء فحص نوع الدم السبت 8 نوفمبر - 6:40 | |
| Blood grouping
Principle
ABO system is the only system in which there is a reciprocal relationship between the antigen on the red cells and the naturally occurring antibodies in the serum.
Routine grouping of donors and patients must therefore include both RBC and serum tests, each serving as check on the other.
The procedure is based on the principle of agglutination of antigen positive red cells in the presence of antibody directed towards the antigen.
Blood grouping is done by 2 methods Forward grouping Reverse grouping | |
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dooa عضو مميز
عدد المساهمات : 270 العمر : 37 تاريخ التسجيل : 05/08/2008
| موضوع: رد: كيفية اجراء فحص نوع الدم السبت 8 نوفمبر - 6:40 | |
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dooa عضو مميز
عدد المساهمات : 270 العمر : 37 تاريخ التسجيل : 05/08/2008
| موضوع: رد: كيفية اجراء فحص نوع الدم السبت 8 نوفمبر - 6:41 | |
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dooa عضو مميز
عدد المساهمات : 270 العمر : 37 تاريخ التسجيل : 05/08/2008
| موضوع: رد: كيفية اجراء فحص نوع الدم السبت 8 نوفمبر - 6:44 | |
| Reverse grouping
In reverse typing, the patient's blood serum is mixed with blood that is known to be type A and type B. Again, the presence of clotting is used to determine the type.
It is also done by 2 methods slide and tube method
here we will describe the the slide method
Label the slide of donor-patient and test identification.
Add 2 drops of test serum on the slide in the corresponding places
Prepare cells for testing of A, B and O (control) groups by pooling 3 samples of each group.
Add 1 drop of 2% pooled A red cell suspension in the place labelled A1.
Add 1 drop of 2% pooled B red cell suspension the place labelled B .
Add 1 drop of 2% pooled O red cell suspension in place labelled O
Mix the serum and cells gently and incubate the test for minimum 15 minutes at room temperature.
And see for agglutination A group B group O group [ You should do forward and reverse grouping and resolve any discrepancies between cell and serum typing tests before the patient's or donor's ABO group is interpreted.
Any missing thing or questions] | |
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شيخ المختبر عضو مميز
عدد المساهمات : 236 تاريخ التسجيل : 11/09/2008
| موضوع: رد: كيفية اجراء فحص نوع الدم الأحد 9 نوفمبر - 12:50 | |
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