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 Calibrating centrifuges for platelets seperation

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عدد المساهمات : 64
العمر : 55
تاريخ التسجيل : 02/10/2008

مُساهمةموضوع: Calibrating centrifuges for platelets seperation   السبت 29 نوفمبر - 8:15

Calibrating centrifuges for Platelets Seperation







Each centrifuge should be calibrated upon receipt & after adjustment or repair .

1- collect from the donor an EDTA tube in addition to the specimen drawn for routine processing .

2- perform a platelet count on the EDTA specimen , if the donor has a platelet count below 133000/mm , this unit of blood shouldn’t be used for calibration .

3- Calculate the number of platelets in the unit of the whole blood (WB) .

Number of platelet in WB = platelet per mm x1000 x volume of WB

4- prepare platelet –rich plasma (PRP) at a selected speed & time .

5- place a temporary clamp on the tubing so that one satellite bag is closed off .

Express platelet rich plasma into the other satellite bag . Seal close to primary bag & disconnect the two satellite bags . Don’t remove the temporary clamp to the satellite bag until the next step .

6- Strip the tubing several times so the tubing contains a representative sample of platelet –rich plasma .

7-Seal off of the tubing & disconnect so the bag of platelet rich plasma remains sterile .

8- Perform a platelet count on the sample of platelet-rich plasma in the segment & calculate the number of platelets in the bag of platelet- rich plasma .

no. of platelets in PRP= platelets count/mm x1000xvolume of RPR .

9- Calculate % yield

% yield = no. of plt in RPR X100%

no. of plt in WB

10- Repeat the above process three or four times with different donors ,using different speeds & times of centrifugation .

11- Compare the yields for each set of centrifuge conditions .

12- Select the shortest time & lowest speed that result in the highest % yield of platelets in platelets rich plasma .

13- Centrifuge the platelets rich plasma at a selected time & speed to prepare platelets concentrate .

14- Express the platelets poor plasma into the second attatched satellite bag & seal the tubing , leaving along section of tubing attatched to platelets concentrate bag .

15- Place the platelets product on an agitator & leave at least for one hour to ensure that the platelets are evenly resuspended . platelets count cannot be performed accuretly on a product immediately after centrifugation .

16- strip the tubing several times , mixing its contents well with the contents of the platelets concentrate bag . let the concentrate flow back into the tubing . seal off a segment of the tubing so that the platelet concentrate bag remains sterile .

17- Perform a platelet count on the platelet concentrate . calculate the number of platelets in the platelets concentrate .

No. of plt in pc = pltcount /mm X 1000 X volume of pc

18- calculate % yield = no. of plt in pc X 100 %

no. of plt in prp

19- Repeat steps 13 through 18 using different speeds & times of centrifugation .

20 –Compare the yields for each set of centrifuge conditions .

21 – Select the shortest time & lowest speed that result in the highest % yield of platelets in the platelets concentrate .

Once each centrifuge has been calibrated , it is not necessary to recalibrate unless there is a problem with the mechanical function of the centrifuge .
بقلم



عادل عبد الحليم السلايمه

أخصائي طب مخبري

مسؤول جودة المياه / بلدية الخليل
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gebriano
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ذكر
عدد المساهمات : 162
العمر : 34
تاريخ التسجيل : 25/11/2008

مُساهمةموضوع: رد: Calibrating centrifuges for platelets seperation   الثلاثاء 16 ديسمبر - 4:50

thanks aloooooooot
v good
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Calibrating centrifuges for platelets seperation
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